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Molecular Biology and PCR Techniques for the Laboratory

A hands on programme covering nucleic acid extraction, conventional PCR and real time quantitative PCR for diagnostic, environmental and research laboratories across Africa. Delegates learn to design primers, prepare reaction mixes, run thermal cyclers and interpret amplification curves. The course stresses contamination control and result reliability in molecular workflows.

What you will be able to do

  • Extract and quantify DNA and RNA from common sample types.
  • Set up conventional and real time PCR reactions with appropriate controls.
  • Design and evaluate primers and probes for target sequences.
  • Interpret amplification and melt curves to confirm valid results.
  • Apply contamination control and workflow separation in a molecular laboratory.
  • Troubleshoot failed reactions, poor efficiency and non specific amplification.

Course outline

  1. 01Nucleic acid structure and extraction methods
  2. 02Principles of PCR and thermal cycling
  3. 03Real time quantitative PCR and detection chemistries
  4. 04Primer and probe design fundamentals
  5. 05Controls, standards and quality checks
  6. 06Contamination control and laboratory zoning
  7. 07Data interpretation and reporting
  8. 08Troubleshooting common PCR failures

Who should attend

Laboratory analysts, medical scientists, technologists and researchers introducing or expanding molecular testing capability.

Dates, venues and fees

DatesVenueDaysFeeCalendar
17 to 19 Aug 2026Online3US$900
24 to 26 Aug 2026Mozambique3US$1,500
31 Aug to 2 Sep 2026Cairo3US$1,500
7 to 9 Sep 2026Accra3US$1,500
14 to 16 Sep 2026Lusaka3US$1,500
21 to 23 Sep 2026Kigali3US$1,500
28 to 30 Sep 2026Nairobi3US$1,500
5 to 7 Oct 2026Durban3R13,000
12 to 14 Oct 2026Johannesburg3R13,000
26 to 28 Oct 2026Mauritius3US$1,500
2 to 4 Nov 2026Dubai3US$1,500
9 to 11 Nov 2026Lagos3US$1,500
16 to 18 Nov 2026Harare3US$1,500
23 to 25 Nov 2026Kampala3US$1,500
30 Nov to 2 Dec 2026Dar es Salaam3US$1,500
7 to 9 Dec 2026Pretoria3R13,000
14 to 16 Dec 2026Cape Town3R13,000

Fees are per delegate. South African venues are priced in rand; other locations and online in US dollars. Fees exclude 15% VAT where applicable. Group bookings of three or more delegates from the same organisation qualify for a reduced rate; contact us for a quotation.

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